22 0 obj endobj stream <> stream 31 0 obj Download Ebook Phytochemical Analysis Methods (PDF) PHYTOCHEMICAL TECHNIQUES - A REVIEW Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents. 24 0 obj 45 0 obj endobj stream A large number of the plants are claimed to possess the antibiotic properties in the traditional system and are also used extensively by the tribal people worldwide. *�U� "����0��� 3�%�e�-���������眼VU� � �z� endstream <> <> stream endstream ?���F{�#�y;��쀷`:�#[�������N{|�m%��V�� K���U�2o౱�e��!eKP�T��Ю���/�$�e���~4�yV���U�U��/wz��o�oƤ��>$�w���5n���p�����M�I�T\�l��O���:������:[��� �5��iR�z�����=8��Dz�����uC���#�ŋ ���*�k�(��-#��ݴKq�͈v�� �A��}�E�wG�� �(���c����� y�0b�k�W��a�߀���ٰZ�2��ޅ�f$�>�� ��C���Ճ@�z_��7}�i�J����7Ut ���x�=��7���x ye4oXN% m�x�h��} ��;��ᓅ����l�8Xw�t�;�СM] A�� ���� b/��v�^��&��M�%��J��-�V�`�w]Fql�����,8M�pr?ý���bY��.kT�moFF�`X�1g�T�9e0�B���D��X�}�N2e%z�J�r|N���|��������@Т��$1� �$E��+����9�槙/�� ����OVӟ�;T5�C���2���[�g.�I58C;N��^���� This method is best suitable for use in case of the thermolabile drugs [3]. endobj Solvent extraction is a widely used method to isolate these bioactive phytochemicals because of the relative simplicity and low cost of the process. Phytochemicals are the individual chemicals from which the plants are made and plants are the key sources of raw material for both pharmaceutical and aromatic industries. <> � �f� 43 0 obj � ��� /Contents 10 0 R>> 39 0 obj <> endstream x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �16Pp�W� The extraction procedures are vital important in analysis of phytochemicals. endstream endobj endobj /Contents 8 0 R>> endstream <> stream Extraction • Is the separation of medicinally active portions of plant (and animal) tissues using selective solvents through standard procedures. x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �1Up�W� endobj endobj <> x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �16Qp�W� endobj stream 56 0 obj � �p� <>/Group <> The extraction procedures are vital important in analysis of phytochemicals There are some traditional extraction methods and novel extraction methods Maceration, percolation and soxhlet extraction methods are prominently used in phytochemical Identification of phytochemicals Plant extracts contains various type of bioactive compounds Phytochemicals: Extraction, Isolation, and Identification of Bioactive Compounds from Plant Extracts Plants (Basel). 61 0 obj <> <> � ��� x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �14Qp�W� <> 14 0 obj <> /Contents 18 0 R>> <> <> 21 0 obj 44 0 obj <>>> /Contents 4 0 R>> /Contents 28 0 R>> 23 0 obj endobj endobj /Contents 68 0 R>> � ��� Therefore, the objective of this paper is to carry out the phytochemical screening, total contents of both phenolics and flavonoids of various solvent extracts of Musa paradisiaca. 1. 52 0 obj endobj Phytochemical analysis for the important chemical constituents from ethanolic extract was carried out. Detection of alkaloids: Extracts were dissolved individually in dilute Hydrochloric acid and filtered. � �x� � � � � ��� 38 0 obj endobj <> endstream /Contents 62 0 R>> <>>>] � �]� 1 0 obj 57 0 obj 66 0 obj <> PRELIMINARY PHYTOCHEMICAL SCREENING The ethanolic extract of Annona squamosa leaves was subjected to preliminary phytochemical screening of various plant constituents (Harborne, 1998; Kokate, 2001). <> <> 70 0 obj endobj endobj <> x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �14Sp�W� endobj 9�y����. 67 0 obj <> stream stream <> endstream 40 0 obj /Contents 52 0 R>> 46 0 obj x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �1�Pp�W� x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �14Pp�W� Analysis used was the method reported by Ejikeme et al. /Contents 6 0 R>> � ��� endstream stream � ��� endobj Maceration, percolation and soxhlet extraction methods are prominently used in phytochemical screening studies. x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �12Qp�W� � �^� <> 59 0 obj 3.5. <> endobj stream 10 0 obj /Contents 26 0 R>> /Contents 58 0 R>> Each wood powder sample (0.30 g) was weighed into a test tube and boiled for 10 minutes in a water bath containing 30 cm 3 of water. endobj endobj endobj 29 0 obj /Length 5824 >> <>/ExtGState<>/XObject<>/ProcSet[/PDF/Text/ImageB/ImageC/ImageI] >>/MediaBox[ 0 0 594.96 841.92] /Contents 4 0 R/Group<>/Tabs/S/StructParents 0>> <> � ��� endobj stream � ��� endobj /Contents 20 0 R>> /Contents 40 0 R>> stream endstream <> Qualitative Analysis of the Phytochemicals of the Wood Sample 2.4.1. endobj <> endobj stream x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �16Vp�W� stream endobj stream 12 0 obj 18 0 obj /Contents 42 0 R>> yield and phytochemicals content) and also to the consequent assays performed. x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �1�Tp�W� endobj In recent years, the new technologies and methods of the extraction, isolation, and structural identification have come forth, which promote the speed of extraction and analysis of phytochemicals. /Contents 32 0 R>> stream 2 0 obj <> <> endobj 0��h�Je�5�\��X�z@���bQ]16�_�Tm^T��jV�����bYQ�\\Eh�KBI���׋/J��uw.��U`/L���^���^��J��{�`*Ȟ���C�4>꙱�[�c! /Contents 56 0 R>> Antioxidant activity of Ocimum sanctum was 35 0 obj Phytochemicals: Extraction Methods, Basic Structures and Mode of Action as Potential Chemotherapeutic Agents 3 degree of basicity varies considerably, depending on the structure of the molecule, and presence and location of the fu nctional groups (Sarker & … x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �12Up�W� endstream x�3R��2�35W(�*T0P�R0T(�Y@���@QC= P A�J��� �14Tp�W� stream ¾Simple traditional to advanced technologies conforming to official <> Hence, this review aim to describe and compare the most commonly used methods based on their principle, strength and limitation to help evaluating the suitability . 33 0 obj stream Decoction This method is used for the extraction of the water soluble and heat stable constituents from crude drug by boiling it in water for 15 minutes, cooling, straining and passing sufficient cold water endstream 63 0 obj Thus, a replacement of these synthetics by antioxidant extractions from various foods has been proposed. Fig 1: Different extraction methods a) cold percolation, b) solvent extraction, c) Supercritical fluid extraction, d) Microwave assisted extraction 2. stream ����)�u�>��)�M�>k*1�~��E�S���x���`��NђI�+��T��|�����r^��+iS[V���e��ZVBf�99���ȹi 9 0 obj 30 0 obj 3 0 obj 51@UV�_��N �S����o�?���s���ս{��A���?o�w_�zP�R�{�ބ�:u���׺s�&t>��g߽z��M����Y���?C�)���_?�?��F�}�0(��Mt�s�ѩ6x���T�V1���il����� =��瞿>���?�S�c?�7��~Ho���3{����ԃ�`c����T��^�wr��e���3�t�����ڻ�O��o��'���-�.�2:�qT!y�&�^�!���N��(+d�ĝds���%9��ٜ�G7h��sN9�~���H���'���5ߒ������'x���Kl���������g��B�W�^��wn����]R���A[�Y^F���M4��#Z��y��Oy>_��f�W��ǜo_��=���.A��יN���eҙ��/�������⅙��}Σ��:����!���Mw4(_Š�Ρ����{Z+%��~���u��W�7B3���,�L0���������F*�xW\���N�2wݡҧ�BGL�9���Q���,R�T*��|u�f�K���o���Ԃ�� Os@kփS>����^p1�nm_=�т2��r'דx���F���My 19 0 obj ���p#Ue�ܮ�X^ov��f�{���� }�ߟևG�fO�^Ξ����Wl��^����j����TR����zg�|��̮���ճk����{�����}}���f��^}{�ޗ��'���9�VT�H��,�Z�!� ��-Q�n3X��z,/+�6h9-m*e��)�_���g^����^[qY�0����l��h��峧�ډ������%�f?��1�fFph�R�oZU��m��{9@�e endobj endobj endobj stream It is now believed that nature has given the cure of every disease in one way or another. endobj 1 0 obj endstream 27 0 obj <> The effects observed could be due to the action of one or more of the phytochemicals present in the leaf extract. <> stream stream endstream � ��� endobj Isolation, and alkaloids used for the identification of cardiac glycosides, phenolic,... 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